Injection:
Article Title: Somatostatin-expressing interneurons induce early NO-driven and late specific astrocyte-mediated vasodilation
Article Snippet: For virus delivery into the primary somatosensory cortex, a small volume of virus (0.5 μL) was infused at a rate of 50 nL/min through a glass pipette connected to a micro-injection pump (Harvard Apparatus, United States) at a depth of 300 and 600 μm about 3–4 weeks before experiments. .. For astrocytic calcium imaging, AAV5-gfaABC1D-cyto-GCaMP6f (Addgene, United States; catalog number: #52925) was injected in SST-ChR2 or wild-type mice. .. For chemogenetic inhibition of SST neurons, AAV5-hSyn-DIO-hM4D(Gi)-mCherry virus (Addgene, #44362) or AAV5-hSyn-DIO-mCherry (control) was injected in SST-cre mice.
Gas Chromatography:
Article Title: Dysfunctional serotonergic neuron-astrocyte signaling in depressive-like states
Article Snippet: Intracranial injections were made using a borosilicate micropipette (World Precision Instuments) at 50 nl/min infusion rate. .. The following viral vectors were used: AAV5-gfaABC1D-cyto-GCaMP6f (Addgene 52925; viral titer 1.3 × 10 13 GC/ml), AAV5-GFAP-hM3Dq-mCherry (Addgene 50478; viral titer 2 × 10 13 GC/ml), AAV8-GFAP104-mCherry (UNC Vector Core; viral titer 2.7 × 10 12 GC/ml), AAV9-hSyn-ChrimsonR-tdTom (UNC Vector Core; viral titer 4.1 × 10 12 GC/ml), AAV5-GFAP-mcherry-cre (UNC Vector Core; viral titer 4.3 × 10 12 GC/ml), AAV5-CAG-flex-iSeroSnFR (Addgene 128486; viral titer 5 x + 10 12 GC/ml, Unitat de Vectors Virals, UAB), AAV5-GFAP-eGFP-WPRE-hGH (Addgene 105549; viral titer 1.3 × 10 13 GC/ml), AAV9-CaMKIIa-eYFP (Penn Vector Core, viral titer 2.55 × 10 12 GC/ml). ..
Article Title: Dysfunctional serotonergic neuron-astrocyte signaling in depressive-like states
Article Snippet: Intracranial injections were made using a borosilicate micropipette (World Precision Instuments) at 50 nl/min infusion rate. .. The following viral vectors were used: AAV5-gfaABC1D-cyto-GCaMP6f (Addgene 52925; viral titer 1.3 x 1013 GC/ml), AAV5-GFAP-hM3Dq-mCherry (Addgene 50478; viral titer 2 x 1013 GC/ml), AAV8-GFAP104-mCherry (UNC Vector Core; viral titer 2.7 x 1012 GC/ml), AAV9-hSyn-ChrimsonR-tdTom (UNC Vector Core; viral titer 4.1 x 1012 GC/ml), AAV5-GFAP-mcherry-cre (UNC Vector Core; viral titer 4.3 x 1012 GC/ml), AAV5-CAG- ex-iSeroSnFR (Addgene 128486; viral titer 5 x+1012 GC/ml, Unitat de Vectors Virals, UAB), AAV5-GFAP-eGFP-WPRE-hGH (Addgene 105549; viral titer 1.3 x 1013 GC/ml). ..
Expressing:
Infection:
Plasmid Preparation:Article Title: Dysfunctional serotonergic neuron-astrocyte signaling in depressive-like states
Article Snippet: Intracranial injections were made using a borosilicate micropipette (World Precision Instuments) at 50 nl/min infusion rate. .. The following viral vectors were used: AAV5-gfaABC1D-cyto-GCaMP6f (Addgene 52925; viral titer 1.3 × 10 13 GC/ml), AAV5-GFAP-hM3Dq-mCherry (Addgene 50478; viral titer 2 × 10 13 GC/ml), AAV8-GFAP104-mCherry (UNC Vector Core; viral titer 2.7 × 10 12 GC/ml), AAV9-hSyn-ChrimsonR-tdTom (UNC Vector Core; viral titer 4.1 × 10 12 GC/ml), AAV5-GFAP-mcherry-cre (UNC Vector Core; viral titer 4.3 × 10 12 GC/ml), AAV5-CAG-flex-iSeroSnFR (Addgene 128486; viral titer 5 x + 10 12 GC/ml, Unitat de Vectors Virals, UAB), AAV5-GFAP-eGFP-WPRE-hGH (Addgene 105549; viral titer 1.3 × 10 13 GC/ml), AAV9-CaMKIIa-eYFP (Penn Vector Core, viral titer 2.55 × 10 12 GC/ml). ..
Article Title: Astrocytes regulate ultra-slow arteriole oscillations via stretch-mediated TRPV4-COX-1 feedback.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-RFP (GOAT) Antibody Rockland Antibodies & Assays Cat# 200-101-379: Lot# 3773; RRID: AB_2744552 Bacterial and virus strains AAV2/5 GfaABC1D mCherry-hPMCA2w/b gift from Baljit Khakh: Addgene Vectors Plasmid #111568 AAV5 GfaABC1D cyto-GCaMP6f (Haustein et al., 2014);: UPenn Vector Core Addgene Vectors Cat#AV-5-52925#52925-AAV5 AAV5 GfaABC1D tdTomato (Tong et al., 2014);: UPenn Vector Core: Addgene Vectors Cat#AV-5-PV310644332-AAV5 Biological samples Acute, living brain slices from mouse and rat This paper N/A Living neocortical brain tissue under a cranial window This paper N/A Chemicals, peptides, and recombinant proteins DNQX disodium salt Tocris BioScience Cat# 2312 Rhod-2/AM Biotium Cat# 50024 FITC-dextran 2000KDa Sigma Aldrich Cat# FD2000S-5G U-46619 Cayman Chemical Cat# 56985-40-1 HC-067047 Tocris Bioscience Cat# 4100 A-967079 Tocris Bioscience Cat# 4716 SC-560 Tocris Bioscience Cat# 1550 HET0016 Cayman Chemical Cat# 75780 Alexa Fluor 488 hydrazide Thermo Fisher Scientific Cat# A-10436 Fluo-4, Pentapotassium Salt, cell impermeant Thermo Fisher Scientific Cat# F14200 4-hydroxy Tamoxifen Cayman Chemical Cat# 17308 Sulforhodamine 101 Sigma Cat# S7635-100MG Experimental models: organisms/strains Mouse: C57BL/6 Charles River https://www.criver.com/products-services/ find-model/c57bl6-mouse?region=24 Rat: genotype Sprague Dawley Rat Charles River https://www.criver.com/products-services/ find-model/sas-sprague-dawley-rat?region=3611 Mouse: Ptgs1(fl/+) Custom made in-house N/A Mouse: Tek-Cre/ERT2 EMMA (via Dr. Craig Brown U Victoria, Canada) Cat# 00715; RRID:IMSR_EM:00715 Mouse: CAG-LSL-Gq-DREADD Jackson Laboratory Cat# 026220; RRID:IMSR_JAX:026220 Mouse: Aldh1l1-Cre/ERT2 Jackson Laboratory Cat# 029655; RRID:IMSR_JAX:029655 Software and algorithms ScanImage Vidrio Technologies https://vidriotechnologies.com/ GraphPad Prism GraphPad Software Inc. https://www.graphpad.com/scientific- software/prism/ RRID:SCR_002798 ImageJ NIH https://imagej.nih.gov/ij/ RRID:SCR_003070 FIJI NIH https://fiji.sc RRID:SCR_002285 Automated structure tracking algorithm Custom https://senselab.med.yale.edu/modeldb/ enterCode?model=266929 (Continued on next page) e1 Cell Reports 36, 109405, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Other Custom perforated cover glass Laser MicroMaching Ltd https://www.lasermicromachining.com Design available upon request Custom titanium head-bar Gordon Lab Design available upon request Custom air supported treadmill (Tran and Gordon, 2015) N/A
Imaging:Article Title: Astrocytic RNA degradation suppresses calcium signaling to support synapse function and restrain anxiety
Article Snippet: .. For astrocyte calcium imaging experiments, 1.2 μL of AAV8-mCherry-Cre (UNC Vector Core) and AAV5-gfaABC1D-cyto-GCaMP6f (52925, Addgene) mixed at a (1:2 ratio) was delivered at stereotaxic coordinates targeting prefrontal cortex (A/P: +1.8, M/L: 0.3, D/V: -1.6 mm), the CA1 area of the hippocampus (A/P: -2.0, M/L: 1.5, D/V: -1.2 mm) and visual cortex (A/P: -2.0, M/L: 1.5, D/V: - 0.75 mm). .. For CalEx calcium imaging the AAV5-GfaABC1D-mcherry-hPMCA2w/b (111568, Addgene) virus was injected at the specified stereotaxic coordinates previously described and experiments were performed 4-6 weeks post-surgery.
Article Title: Somatostatin-expressing interneurons induce early NO-driven and late specific astrocyte-mediated vasodilation
Article Snippet: For virus delivery into the primary somatosensory cortex, a small volume of virus (0.5 μL) was infused at a rate of 50 nL/min through a glass pipette connected to a micro-injection pump (Harvard Apparatus, United States) at a depth of 300 and 600 μm about 3–4 weeks before experiments. .. For astrocytic calcium imaging, AAV5-gfaABC1D-cyto-GCaMP6f (Addgene, United States; catalog number: #52925) was injected in SST-ChR2 or wild-type mice. .. For chemogenetic inhibition of SST neurons, AAV5-hSyn-DIO-hM4D(Gi)-mCherry virus (Addgene, #44362) or AAV5-hSyn-DIO-mCherry (control) was injected in SST-cre mice.
Virus:Article Title: Astrocytes regulate ultra-slow arteriole oscillations via stretch-mediated TRPV4-COX-1 feedback.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-RFP (GOAT) Antibody Rockland Antibodies & Assays Cat# 200-101-379: Lot# 3773; RRID: AB_2744552 Bacterial and virus strains AAV2/5 GfaABC1D mCherry-hPMCA2w/b gift from Baljit Khakh: Addgene Vectors Plasmid #111568 AAV5 GfaABC1D cyto-GCaMP6f (Haustein et al., 2014);: UPenn Vector Core Addgene Vectors Cat#AV-5-52925#52925-AAV5 AAV5 GfaABC1D tdTomato (Tong et al., 2014);: UPenn Vector Core: Addgene Vectors Cat#AV-5-PV310644332-AAV5 Biological samples Acute, living brain slices from mouse and rat This paper N/A Living neocortical brain tissue under a cranial window This paper N/A Chemicals, peptides, and recombinant proteins DNQX disodium salt Tocris BioScience Cat# 2312 Rhod-2/AM Biotium Cat# 50024 FITC-dextran 2000KDa Sigma Aldrich Cat# FD2000S-5G U-46619 Cayman Chemical Cat# 56985-40-1 HC-067047 Tocris Bioscience Cat# 4100 A-967079 Tocris Bioscience Cat# 4716 SC-560 Tocris Bioscience Cat# 1550 HET0016 Cayman Chemical Cat# 75780 Alexa Fluor 488 hydrazide Thermo Fisher Scientific Cat# A-10436 Fluo-4, Pentapotassium Salt, cell impermeant Thermo Fisher Scientific Cat# F14200 4-hydroxy Tamoxifen Cayman Chemical Cat# 17308 Sulforhodamine 101 Sigma Cat# S7635-100MG Experimental models: organisms/strains Mouse: C57BL/6 Charles River https://www.criver.com/products-services/ find-model/c57bl6-mouse?region=24 Rat: genotype Sprague Dawley Rat Charles River https://www.criver.com/products-services/ find-model/sas-sprague-dawley-rat?region=3611 Mouse: Ptgs1(fl/+) Custom made in-house N/A Mouse: Tek-Cre/ERT2 EMMA (via Dr. Craig Brown U Victoria, Canada) Cat# 00715; RRID:IMSR_EM:00715 Mouse: CAG-LSL-Gq-DREADD Jackson Laboratory Cat# 026220; RRID:IMSR_JAX:026220 Mouse: Aldh1l1-Cre/ERT2 Jackson Laboratory Cat# 029655; RRID:IMSR_JAX:029655 Software and algorithms ScanImage Vidrio Technologies https://vidriotechnologies.com/ GraphPad Prism GraphPad Software Inc. https://www.graphpad.com/scientific- software/prism/ RRID:SCR_002798 ImageJ NIH https://imagej.nih.gov/ij/ RRID:SCR_003070 FIJI NIH https://fiji.sc RRID:SCR_002285 Automated structure tracking algorithm Custom https://senselab.med.yale.edu/modeldb/ enterCode?model=266929 (Continued on next page) e1 Cell Reports 36, 109405, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Other Custom perforated cover glass Laser MicroMaching Ltd https://www.lasermicromachining.com Design available upon request Custom titanium head-bar Gordon Lab Design available upon request Custom air supported treadmill (Tran and Gordon, 2015) N/A
Recombinant:Article Title: Astrocytes regulate ultra-slow arteriole oscillations via stretch-mediated TRPV4-COX-1 feedback.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-RFP (GOAT) Antibody Rockland Antibodies & Assays Cat# 200-101-379: Lot# 3773; RRID: AB_2744552 Bacterial and virus strains AAV2/5 GfaABC1D mCherry-hPMCA2w/b gift from Baljit Khakh: Addgene Vectors Plasmid #111568 AAV5 GfaABC1D cyto-GCaMP6f (Haustein et al., 2014);: UPenn Vector Core Addgene Vectors Cat#AV-5-52925#52925-AAV5 AAV5 GfaABC1D tdTomato (Tong et al., 2014);: UPenn Vector Core: Addgene Vectors Cat#AV-5-PV310644332-AAV5 Biological samples Acute, living brain slices from mouse and rat This paper N/A Living neocortical brain tissue under a cranial window This paper N/A Chemicals, peptides, and recombinant proteins DNQX disodium salt Tocris BioScience Cat# 2312 Rhod-2/AM Biotium Cat# 50024 FITC-dextran 2000KDa Sigma Aldrich Cat# FD2000S-5G U-46619 Cayman Chemical Cat# 56985-40-1 HC-067047 Tocris Bioscience Cat# 4100 A-967079 Tocris Bioscience Cat# 4716 SC-560 Tocris Bioscience Cat# 1550 HET0016 Cayman Chemical Cat# 75780 Alexa Fluor 488 hydrazide Thermo Fisher Scientific Cat# A-10436 Fluo-4, Pentapotassium Salt, cell impermeant Thermo Fisher Scientific Cat# F14200 4-hydroxy Tamoxifen Cayman Chemical Cat# 17308 Sulforhodamine 101 Sigma Cat# S7635-100MG Experimental models: organisms/strains Mouse: C57BL/6 Charles River https://www.criver.com/products-services/ find-model/c57bl6-mouse?region=24 Rat: genotype Sprague Dawley Rat Charles River https://www.criver.com/products-services/ find-model/sas-sprague-dawley-rat?region=3611 Mouse: Ptgs1(fl/+) Custom made in-house N/A Mouse: Tek-Cre/ERT2 EMMA (via Dr. Craig Brown U Victoria, Canada) Cat# 00715; RRID:IMSR_EM:00715 Mouse: CAG-LSL-Gq-DREADD Jackson Laboratory Cat# 026220; RRID:IMSR_JAX:026220 Mouse: Aldh1l1-Cre/ERT2 Jackson Laboratory Cat# 029655; RRID:IMSR_JAX:029655 Software and algorithms ScanImage Vidrio Technologies https://vidriotechnologies.com/ GraphPad Prism GraphPad Software Inc. https://www.graphpad.com/scientific- software/prism/ RRID:SCR_002798 ImageJ NIH https://imagej.nih.gov/ij/ RRID:SCR_003070 FIJI NIH https://fiji.sc RRID:SCR_002285 Automated structure tracking algorithm Custom https://senselab.med.yale.edu/modeldb/ enterCode?model=266929 (Continued on next page) e1 Cell Reports 36, 109405, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Other Custom perforated cover glass Laser MicroMaching Ltd https://www.lasermicromachining.com Design available upon request Custom titanium head-bar Gordon Lab Design available upon request Custom air supported treadmill (Tran and Gordon, 2015) N/A
Software:Article Title: Astrocytes regulate ultra-slow arteriole oscillations via stretch-mediated TRPV4-COX-1 feedback.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-RFP (GOAT) Antibody Rockland Antibodies & Assays Cat# 200-101-379: Lot# 3773; RRID: AB_2744552 Bacterial and virus strains AAV2/5 GfaABC1D mCherry-hPMCA2w/b gift from Baljit Khakh: Addgene Vectors Plasmid #111568 AAV5 GfaABC1D cyto-GCaMP6f (Haustein et al., 2014);: UPenn Vector Core Addgene Vectors Cat#AV-5-52925#52925-AAV5 AAV5 GfaABC1D tdTomato (Tong et al., 2014);: UPenn Vector Core: Addgene Vectors Cat#AV-5-PV310644332-AAV5 Biological samples Acute, living brain slices from mouse and rat This paper N/A Living neocortical brain tissue under a cranial window This paper N/A Chemicals, peptides, and recombinant proteins DNQX disodium salt Tocris BioScience Cat# 2312 Rhod-2/AM Biotium Cat# 50024 FITC-dextran 2000KDa Sigma Aldrich Cat# FD2000S-5G U-46619 Cayman Chemical Cat# 56985-40-1 HC-067047 Tocris Bioscience Cat# 4100 A-967079 Tocris Bioscience Cat# 4716 SC-560 Tocris Bioscience Cat# 1550 HET0016 Cayman Chemical Cat# 75780 Alexa Fluor 488 hydrazide Thermo Fisher Scientific Cat# A-10436 Fluo-4, Pentapotassium Salt, cell impermeant Thermo Fisher Scientific Cat# F14200 4-hydroxy Tamoxifen Cayman Chemical Cat# 17308 Sulforhodamine 101 Sigma Cat# S7635-100MG Experimental models: organisms/strains Mouse: C57BL/6 Charles River https://www.criver.com/products-services/ find-model/c57bl6-mouse?region=24 Rat: genotype Sprague Dawley Rat Charles River https://www.criver.com/products-services/ find-model/sas-sprague-dawley-rat?region=3611 Mouse: Ptgs1(fl/+) Custom made in-house N/A Mouse: Tek-Cre/ERT2 EMMA (via Dr. Craig Brown U Victoria, Canada) Cat# 00715; RRID:IMSR_EM:00715 Mouse: CAG-LSL-Gq-DREADD Jackson Laboratory Cat# 026220; RRID:IMSR_JAX:026220 Mouse: Aldh1l1-Cre/ERT2 Jackson Laboratory Cat# 029655; RRID:IMSR_JAX:029655 Software and algorithms ScanImage Vidrio Technologies https://vidriotechnologies.com/ GraphPad Prism GraphPad Software Inc. https://www.graphpad.com/scientific- software/prism/ RRID:SCR_002798 ImageJ NIH https://imagej.nih.gov/ij/ RRID:SCR_003070 FIJI NIH https://fiji.sc RRID:SCR_002285 Automated structure tracking algorithm Custom https://senselab.med.yale.edu/modeldb/ enterCode?model=266929 (Continued on next page) e1 Cell Reports 36, 109405, August 3, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Other Custom perforated cover glass Laser MicroMaching Ltd https://www.lasermicromachining.com Design available upon request Custom titanium head-bar Gordon Lab Design available upon request Custom air supported treadmill (Tran and Gordon, 2015) N/A
|
Addgene inc is a verified supplier 